The Power of RNA-seq
Wageningen, June 11-13 2018
Program
Evaluation form
Day 1
Hand outs
1a Introduction
1a NGS
1b RNAseq
1c Assembly, annotation, quantification
Practicals
1b Mapping
1c Quantification
Data files
ST_1.fastq
SRR5304927_1.fastq
SRR5304927.zip
ST_vs_HT.csv
Day 2
Hand outs
2a Differential Expression
2b Clustering
Practicals
2a Differential Expression
2b Clustering
(
answers
)
Data files
kallisto.zip
km.txt
hc.txt
wgcna_edges.txt
wgcna_nodes.txt
Day 3
Hand outs
3a Interpretation
Tulip and Lily transcriptomes
Practicals
3a Interpretation
Links
Linux command-line tutorial (interactive)
Sleuth walkthroughs
Transcript-level expression analysis of RNA-seq experiments with HISAT, StringTie and Ballgown
Analyzing RNA-seq data with DESeq2
De novo transcript sequence reconstruction from RNA-seq using the Trinity platform for reference generation and analysis