Intro
What...?
Available
Aim
Clone Example
E and B sites
Transformation
Ligation products
pGT4 in detail
Recomb in detail
Recombs Selection
Minipreps
Gel
Blot 1
Blotting
Labeling
Blot 2
Label Detection
Blot 3
Blot and gel
AE1
AE1 (cont.)
AE2
AE2 (cont.)
PCR

Additional experiment 1


pGT4


pGT4ΔB

You'll make a mutation in pGT4: the BamHI site will be removed.
The resulting mutant plasmid vecor pGT4ΔB will be checked for the absence of the BamHI site.
The disruption of the tet-resistance gene will be demonstrated.

The Simple Cloning Lab          Cloning Course