Intro What...? Available Aim Clone Example E and B sites Transformation Ligation products pGT4 in detail Recomb in detail Recombs Selection Minipreps Gel Blot 1 Blotting Labeling Blot 2 Label Detection Blot 3 Blot and gel AE1 AE1 (cont.) AE2 AE2 (cont.) PCR
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Additional experiment 1
pGT4
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pGT4ΔB
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You'll make a mutation in pGT4:
the BamHI site will be removed.
The resulting mutant plasmid vecor
pGT4ΔB will be checked for the
absence of the BamHI site.
The disruption of the tet-resistance gene will be demonstrated. |
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