Intro
What...?
Available
Aim
Clone Example
E and B sites
Transformation
Ligation products
pGT4 in detail
Recomb in detail
Recombs Selection
Minipreps
Gel
Blot 1
Blotting
Labeling
Blot 2
Label Detection
Blot 3
Blot and gel
AE1
AE1 (cont.)
AE2
AE2 (cont.)
PCR

Additional steps

The steps for the additional experiment of groups A (column on the right in the table below)
will be done in between the steps for the Lambda library construction (left column).

basic flow chart

additions/modifications for A groups

The Simple Cloning Lab          Cloning Course