Intro What...? Available Aim Clone Example E and B sites Transformation Ligation products pGT4 in detail Recomb in detail Recombs Selection Minipreps Gel Blot 1 Blotting Labeling Blot 2 Label Detection Blot 3 Blot and gel AE1 AE1 (cont.) AE2 AE2 (cont.) PCR
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Additional experiment 2
pGTλ3758
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pGTλ3758ΔH
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The HindIII fragment within the Lambda insert
of one of the clones will be removed, to create the
mutant pGTλ3758ΔH.
The mutation (deletion of the HindIII
fragment) will be checked by the change of
the insert size. |
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