Intro
What...?
Available
Aim
Clone Example
E and B sites
Transformation
Ligation products
pGT4 in detail
Recomb in detail
Recombs Selection
Minipreps
Gel
Blot 1
Blotting
Labeling
Blot 2
Label Detection
Blot 3
Blot and gel
AE1
AE1 (cont.)
AE2
AE2 (cont.)
PCR

Additional experiment 2


pGTλ3758


pGTλ3758ΔH

The HindIII fragment within the Lambda insert of one of the clones will be removed, to create the mutant pGTλ3758ΔH.
The mutation (deletion of the HindIII fragment)  will be checked by the change of the insert size.

The Simple Cloning Lab          Cloning Course