Intro
What...?
Available
Aim
Clone Example
E and B sites
Transformation
Ligation products
pGT4 in detail
Recomb in detail
Recombs Selection
Minipreps
Gel
Blot 1
Blotting
Labeling
Blot 2
Label Detection
Blot 3
Blot and gel
AE1
AE1 (cont.)
AE2
AE2 (cont.)
PCR

Aim of the Cloning Course

Aim:

to get a well-defined set of bacterial clones, a "mini library" of Lambda.

 

A "clone":

a bacterium, harboring a plasmid, containing a foreign DNA fragment (a recombinant plasmid)

The Simple Cloning Lab          Cloning Course