Intro What...? Available Aim Clone Example E and B sites Transformation Ligation products pGT4 in detail Recomb in detail Recombs Selection Minipreps Gel Blot 1 Blotting Labeling Blot 2 Label Detection Blot 3 Blot and gel AE1 AE1 (cont.) AE2 AE2 (cont.) PCR
Aim of the Cloning Course
to get a well-defined set of bacterial clones, a "mini library" of Lambda.
A "clone":
a bacterium, harboring a plasmid, containing a foreign DNA fragment (a recombinant plasmid)
The Simple Cloning Lab Cloning Course