Intro
What...?
Available
Aim
Clone Example
E and B sites
Transformation
Ligation products
pGT4 in detail
Recomb in detail
Recombs Selection
Minipreps
Gel
Blot 1
Blotting
Labeling
Blot 2
Label Detection
Blot 3
Blot and gel
AE1
AE1 (cont.)
AE2
AE2 (cont.)
PCR

The stained blot

The purple precipitate shows where the AP is,
which is where the antibody is,
which is where the DIG label is,
which is where the probe is,
which shows the DNA fragments containing probe sequences.

The Simple Cloning Lab          Cloning Course