The blotting
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Transfer solution 8 is taken up by the stack of paper towels 3. The DNA is transferred by the flow that goes through wick 6, gel 5 and nylon filter (or "blotting membrane") 4. DNA molecules cannot pass that filter and are bound to it. Solution 8 has a very high pH and will denature the DNA. So, the DNA pattern of the gel is copied into a pattern of single-stranded DNAs on the filter. This filter, now called a "blot", can be used in a subsequent hybridisation experiment.. |
