Probe labeling
A DNA fragment that is to be used as a
hybridisation probe, is copied and labelled with a DNA polymerase and labelled
nucleotides. In this course, the label is
Digoxygenin ("DIG"). DIG can easily
be detected by anti-DIG antibodies.
note:
some groups will copy and label a fragment purified from an agarose gel,
others
will copy and label a PCR-amplified insert from an already available Lambda
clone...
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