Intro
What...?
Available
Aim
Clone Example
E and B sites
Transformation
Ligation products
pGT4 in detail
Recomb in detail
Recombs Selection
Minipreps
Gel
Blot 1
Blotting
Labeling
Blot 2
Label Detection
Blot 3
Blot and gel
AE1
AE1 (cont.)
AE2
AE2 (cont.)
PCR

The recombinant
compared to pGT4

pGT4

A recombinant plasmid in more detail.

In a recombinant plasmid, by insertion of the Lambda DNA fragment, the tetracyclin resistance gene is disrupted.

So, transformants are selected by growth on ampicillin-containing medium.
Recombinants among the transformants are (negatively) selected by growth on tetracyclin-containing
medium..
Note:
In the Simple Cloning Lab, the name of the recombinant plasmid or clone shows the size of the Lambda insert (the red part in the picture on the right). For example, plasmid pGTl1120 is pGT4 with the 1120 basepairs EcoRI-BamHI fragment of lambda DNA inserted ..

The Simple Cloning Lab          Cloning Course