Intro What...? Available Aim Clone Example E and B sites Transformation Ligation products pGT4 in detail Recomb in detail Recombs Selection Minipreps Gel Blot 1 Blotting Labeling Blot 2 Label Detection Blot 3 Blot and gel AE1 AE1 (cont.) AE2 AE2 (cont.) PCR
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The recombinant
compared to pGT4
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pGT4
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A recombinant plasmid in more detail.
In a recombinant plasmid, by insertion of the Lambda DNA
fragment,
the tetracyclin resistance gene is
disrupted.
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So, transformants
are selected by growth on ampicillin-containing medium.
Recombinants among the transformants are (negatively) selected by
growth on tetracyclin-containing medium.. |
Note:
In the Simple Cloning Lab, the name of the recombinant plasmid or clone
shows the size of the Lambda insert (the red part in the picture on the
right). For example, plasmid pGTl1120 is pGT4
with the 1120 basepairs EcoRI-BamHI fragment of lambda DNA inserted .. |
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