Transformation
The bacteria take up the DNA very inefficiently.
And not more than one molecule per bacterium is taken up, if any..
We will analyse individual colonies formed by the bacteria on an agar plate containing the antibiotic ampicillin, to select for presence of a pGT4(-derived) plasmid..
The agar plate may look like this, after overnight growth:
Each colony represents a single clone,
harbouring a plasmid originating from one DNA molecule from the ligation reaction mixture.
The bacteria are transformed by the uptake of
the DNA.
We hope to find recombinants among those
transformants..
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