Intro What...? Available Aim Clone Example E and B sites Transformation Ligation products pGT4 in detail Recomb in detail Recombs Selection Minipreps Gel Blot 1 Blotting Labeling Blot 2 Label Detection Blot 3 Blot and gel AE1 AE1 (cont.) AE2 AE2 (cont.) PCR
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MOB20306
What are you going to do?
- 9 half days at the computer
- you will learn about the results of 6 virtual students
doing a Cloning Course in the
Simple Cloning Lab
- On the last day:
a 2.5 hours TEST, each student
individually,
on paper, with the internet accessible (!!),
with multiple answer questions about the Simple Cloning Lab only.
- 9 half days in the wet lab,
actually doing the cloning experiments of the
Simple Cloning Lab yourself..
- You will have to write a report on the wet part
note:
final mark for the practical course:
- 25%: TEST
- 25%: computer sessions performance and wet lab performance
- 50%: wet lab report
All three parts of the practical can compensate for each other
to get a
final practicals mark of at least 5.5
note: there will be no possibility to re-do the TEST..
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