Intro
What...?
Available
Aim
Clone Example
E and B sites
Transformation
Ligation products
pGT4 in detail
Recomb in detail
Recombs Selection
Minipreps
Gel
Blot 1
Blotting
Labeling
Blot 2
Label Detection
Blot 3
Blot and gel
AE1
AE1 (cont.)
AE2
AE2 (cont.)
PCR

Gene Technology MOB20306

What are you going to do?

  • 9 half days at the computer
    • you will learn about the results of 6 virtual students
      doing a Cloning Course in the Simple Cloning Lab
    • On the last day:
      a 2.5 hours TEST, each student individually,
      on paper, with the internet accessible (!!),
      with multiple answer questions about the Simple Cloning Lab only.
  • 9 half days in the wet lab,
    actually doing the cloning experiments of the Simple Cloning Lab yourself..
    • You will have to write a report on the wet part

 

note:
final mark
for the practical course:

  • 25%: TEST
  • 25%: computer sessions performance and wet lab performance
  • 50%: wet lab report


All three parts of the practical can compensate for each other
to get a final practicals mark of at least 5.5
            note: there will be no possibility to re-do the TEST..

 

The Simple Cloning Lab          Cloning Course