Intro
What...?
Available
Aim
Clone Example
E and B sites
Transformation
Ligation products
pGT4 in detail
Recomb in detail
Recombs Selection
Minipreps
Gel
Blot 1
Blotting
Labeling
Blot 2
Label Detection
Blot 3
Blot and gel
AE1
AE1 (cont.)
AE2
AE2 (cont.)
PCR The Gene Technology practicals = a basic cloning course
What is the Cloning Course about?
- Cloning of
bacteriophage Lambda DNA fragments
in E.coli
using plasmid vector pGT4.
