EMBOSS explorer

eprimer3

Pick PCR primers and hybridization oligos (read the manual)

Only required fields are visible. (show optional fields)

Input section

Select an input sequence. Use one of the following three fields:

  1. To access a sequence from a database, enter the USA here:
  2. To upload a sequence from your local computer, select it here:
  3. To enter the sequence data manually, type here:
Additional section
Program options
Sequence options
Primer options
Product options
Internal oligo input
Internal oligo options
Advanced section
Primer penalty weights
Output section
Run section
Email address: If you are submitting a long job and would like to be informed by email when it finishes, enter your email address here.