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pGTλclones |
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pGTλclonesAll Lambda clones made in the Simple Cloning Lab have plasmids with a similar structure (see below, circle on the right) :
Between the unique EcoR I and BamH I sites in pGT4 (see
above, circle on the left) a BamHI-EcoRI fragment from Lambda DNA (shown
in red) is inserted using the enzyme ligase ("ligated"). Since
recombination of (parts of) the vector (pGT4) and donor
(Lambda DNA) has occurred, the plasmids are called recombinant
plasmids (pGTλ plasmids). Note:
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