Simple Cloning Lab

Overview
Digestions
pGT4ΔB
Electrophoresis
DNA clean-up
Ligation
Fragment isolation
Competent cells
Transformation
Recombinants
pGTλ3758ΔH
Miniprepping
Blotting
Probe Labeling
Hybridisation
Probe Detection
PCR

The Simple Cloning Lab is made to prepare you for the wet lab experiments of Gene Technology MOB20306 and consists of three different websites:

Simple Cloning Lab is the main website.
SCL Questions containes questions and assignments for the Sections of the Simple Cloning Lab.
SCL Resources gives you additional information to find the answers.

To "do" this virtual lab, start in the Simple Cloning Lab with reading and understanding the Sections indicated on the left, and try to answer to the accompanying questions in SCL Questions...

  • Simple Cloning Lab ('the grey site', where you are at this very moment..)
  • SCL Resources ("the orange site")
  • SCL Questions  ("the blue Drupal site")
    accessable through log-in on BlackBoard
    and then:
    MOB20306 Gene Technology
    > Course Documents
    > Simple Cloning Lab questions

Open these three sites in separate browser tabs,
and never use those scrolbars, but use (Shift-) spacebar for easy up and down scrolling instead!!

Note: to come back to this page, click the SCL logo on the top left of any other grey site page)