In the Simple Cloning Lab, students are trying to make a
bacteriophage Lambda DNA library, a collection of bacteriophage
Lambda DNA fragments cloned into the bacterium Escherichia coli. This should result in a set of
E.coli clones, each harbouring a
distinct recombinant plasmid. To construct the library, plasmid pGT4 is used as a vector.
See the flowschemes below
Also attempts are made to construct (examples of) mutants: a mutant vector plasmid (pGT4ΔB) and a
mutant version of one of the library clones (pGTλ3758ΔH) are
made.
See below for a flowscheme for the bacteriophage Lambda DNA library
(use your mouse to hover over the scheme):
Steps 1, 2, [3], 4, 5, 6, 7, 8, 9 could be called
library construction,
steps [3], 10, 11, 12, 13, 14 could be called library screening.
