Intro
What...?
Available
Aim
Clone Example
E and B sites
Transformation
Ligation products
pGT4 in detail
Recomb in detail
Recombs Selection
Minipreps
Gel
Blot 1
Blotting
Labeling
Blot 2
Label Detection
Blot 3
Blot and gel
AE1
AE1 (cont.)
AE2
AE2 (cont.)
PCR

The starting material

available:

2 purified DNAs:
  • pGT4:
    plasmid vector DNA, ~3.9 kb
  • Lambda DNA, ~48 kb

and a bacterial host strain

The Simple Cloning Lab          Cloning Course