Intro
What...?
Available
Aim
Clone Example
E and B sites
Transformation
Ligation products
pGT4 in detail
Recomb in detail
Recombs Selection
Minipreps
Gel
Blot 1
Blotting
Labeling
Blot 2
Label Detection
Blot 3
Blot and gel
AE1
AE1 (cont.)
AE2
AE2 (cont.)
PCR

The miniprep gel

A black-and-white photograph sketch of the gel showing  digested minipreps 1, 2 and 3.
The lane 4 on the right shows a Lambda  BamH I plus EcoR I DNA digest.
The inserts can easily be identified:
Clone 1 contains fragment d
Clone 2 contains fragment f
Clone 3 contains fragment e
Most of the times the result is not this clear and additional information about the clones is needed. For this, you need to do a hybridisation experiment using a "blot" made from this gel, and a labelled "probe" containing Lambda DNA.

The Simple Cloning Lab          Cloning Course