Intro What...? Available Aim Clone Example E and B sites Transformation Ligation products pGT4 in detail Recomb in detail Recombs Selection Minipreps Gel Blot 1 Blotting Labeling Blot 2 Label Detection Blot 3 Blot and gel AE1 AE1 (cont.) AE2 AE2 (cont.) PCR
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The miniprep gel
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A black-and-white photograph sketch of the
gel showing digested minipreps 1, 2 and 3.
The lane 4 on the right shows a Lambda BamH I plus EcoR I DNA
digest.
The inserts can easily be identified:
Clone 1 contains fragment d
Clone 2 contains fragment f
Clone 3 contains fragment e |
Most of the times the result is not this
clear and additional information about the clones is needed. For this,
you need to do a hybridisation
experiment using a "blot" made
from this gel, and a labelled "probe"
containing Lambda DNA. |
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